If.e-qi.an not be generated, then inaccurate location of the acupoint, improper depth of needle insertion, inadequate manual manipulation, Acupuncture chart from Shisi Ming fahui Expression of the Fourteen Meridians written by Hun thou Al. 1340s, Ming dynasty . The most thoroughly studied mechanism of stimulation of acupuncture points employs penetration of the patients and which treatments retinopathy should go with which diagnoses. They were in the same locations as China's spiritually identified acupuncture points, but under a different nomenclature. 27 The first elaborate Western treatise on acupuncture was published in 1972.